Assessment of Antimicrobial by Nanocomposite of Bacterial Cellulose Loaded with Nanoparticles Sodium Alginate and Chitosan

Abstract

Antimicrobial membrane disc of BC with sodium alginate and chitosan were fabricated through doping nanoparticles as antibacterial agents. . Nanoparticles was densely arrayed inside BC fibers composites as confirmed by field emission scanning electron microscopy (FE-SEM). Fourier transform-infrared (FT-IR) spectra of the BC membranes exhibited characteristic peaks for specific functional groups of ch and sd alg beside swelling test and antimicrobial property of these nanoparticles sodium alginate and Chitosan doped cellulose membrane against E. coli and S. aureus was evaluated by the diffusion disc method. Effects of antimicrobial agent quantity, volume-related properties (nanoparticles) on the antimicrobial activity of cellulose membrane were studied. Antimicrobial activity was observed Chitosan nanoparticles were compared when compared with sodium alginate. Maximum E. coli inhibition 85% was achieved with only 5% of amphetamine from the nanoparticles chitosan in cellulose membrane. The resulting bio composites membrane have high antibiotic activity against Staphylococcus aureus. These membranes can potentially be used in medicine as a wound healing.

Keywords: Bacterial cellulose, chitosan, nanoparticles, antibacterial activity, Staphylococcus aureus E. coli.