Plant Viruses Detection and Diagnosis Based on Polymerase Chain Reaction Techniques: Review

Abstract

Plant virus diseases result in the loss of billions of dollars annually by limiting plant production quantity and quality in the world. Among different strategies adapted for plant virus disease management, proper diagnosis and detection are the most important and essential strategies for the development of appropriate control measures. The current advanced techniques developed for the detection of plant viruses provided the chance to take practical managemental actions timely. Nowadays one of the most advanced diagnosis methods, polymerase chain reaction (PCR) is used extensively for the detection and identification of plant viruses. PCR is the advanced method that allows the specific amplification and hence detection of target DNA sequences from a mixture of nucleic acid extract in which specific amplification of targeted fragments of a single or a few copies of source DNA material is achieved within a few hours. The PCR method copies each piece of DNA fragments through all the cycles that leading to an exponential doubling of copies over time. Several modifications of PCR methods have been developed to boost the effectiveness of the method in diagnostic settings based on their applications. Reverse transcriptase PCR, immunocapture PCR, Multiplex PCR, co-operational PCR, and real-time PCR are the common and widely used types of PCR variants.

Keywords: Detection techniques; PCR; Plant virus

DOI: 10.7176/ALST/86-02

Publication date:March 31^st 2021