Chronic Consumption of Abelmoschus Esculentus and Piper Guineense Induce Testicular-Toxicity in Wistar Rats, Histopathological Finding

Abstract

Histopathology of the Testes is one of the parameters used in assessing its micro-structural integrity. In this study, the effect of the oral chronic consumption of 500mg/kg of Abelmoschus esculentus and 20mg/kg of piper guineense on the Testes of wistar rats was assessed. Twenty adult wistar male rats weighing (123-207g), divided into four groups 1, II, III &1V, group 1 as control and groups II, III &IV as experimental groups. The rats in the control group were administered with distilled water, while rats in group II and III were administered with 500mg/kg of Abelmoschus esculentus and 20mg/kg of piper guineense respectively. Group IV received a combination of the two extracts. After 28days of administration of extracts, animals were sacrificed Testes was extracted and processed to paraffin section, cut at 5micron, stained, and observed histopathologically under light microscope. Result showed numerous atrophied and damaged seminiferous tubules, degenerated myoid cells, spermatogenic lining cells, spermatogonia, spermatocytes, spermatids, spermatozoa and lumen filled with semen, degenerated interstitial cells of leydig and interstitial fibrosis against the background of connective tissues with marked area of necrosis in group II and III and IV as compared to the control group 1. Statistical value in the weight of the body and testes showed significant value (p<0.05) compared to control.

In conclusion, Abelmoschus esculentus and Piper guineense has severe toxicity effect on the testes of albino wistar rats.

Keywords: Abelmoschus esculentus, piper guineense, Histopathology, Testes and wistar rat.