Detection of Adhesion Genes and Slim Production among Staphylococcus Aureus and Staphylococcus Epidermidis Isolated from Hemodialysis Patients

Abstract

The presence of the ica loci and adhesins genes in clinical Staphylococcus aureus and Staphylococcus epidermidis strains were considered important factors of virulence. In this study, one isolate of S. epidermidis and six isolates of S. aureus were isolated from cases of septicemia in hemodialysis patients and were investigated for slime production using Congo red agar methods (CRA). Biofilm production of S. epidermidis and S. aureus by using (CRA) plate revealed that all seven isolates were slime producers.

In order to detect the adhesion genes (clf A, fnb A and cna) and presence of slime genes       (ica A and ica D) genes polymerase chain reaction was used. All seven isolates were ica A and ica D positive. While the (clf A, fnb A and cna) were positive in S. aureus and negative in S. epidermidis. This study confirms the presence of clf A, fnb A and can and ica A/D genes in the majorty studies S. aureus strains isolates from different sites of infection.

Keywords:Staphylococcus aureus,S.epidermidis,Biofilm production,Adhesion genes