Electrochemical Detection of Glucose by Using Glucose Oxidase Modified Platinum Electrode

Abstract

In this research, the cysteamine self-assembled monolayers (SAM), is required to modify surface of pt electrode. Now, by using the method of chemical binding and electrochemical deposition the surface of platinum electrode was modified by glucose oxidase enzyme. Platinum plate (0.5cm×0.5cm), platinum rod and silver or silver chloride electrodes act as working, auxiliary and reference electrodes, individually. The manufactured of pt. /GOD electrode described by electrochemical impedance spectroscopy (EIS). The glucose oxidase modified platinum electrode was used for detection of glucose by Amperometric measurement. The amperometric response of the sensor was observed under some factors i.e. pH, Concentration, Temperature and applied potential. Detection of glucose can be taken by oxidation of enzymatically formed H2O2 at 0.8 V. A high current response was occur at pH 7.5 and potential +0.8 V. Enzyme is stronger at high temperature because of micro environment but at 28 °C biosensor show good response and this is working temperature.  The stability of the enzyme electrode was also deliberated. Moreover, the performance of the glucose oxidase enzyme was reserved about 59.9% after a time of 15 days. These factors show pt/GOD electrode is good or successful biosensor for detection of glucose.

Index Terms— Biosensor, Cysteamine self-assembled, Glucose oxidase, Glucose, Immobilization,

DOI: 10.7176/CMR/11-4-03

Publication date: April 30^th 2019