A Quantitative Estimation of Phytochemical, Anti-Diabetic and Anti-Oxidant Activity of Crude Extract of Root of Jurinea Dolomiaea Boiss

Abstract

The therapeutic efficacy of Jurinea dolomiaea (root) was selected to analyse the anti-diabetic and anti-oxidant activities of their crude plant extract. The plant samples were extracted from polar solvents viz. n-hexane, ethyl acetate, methanol and water. Further crude extracts were screened for the presence of phytochemicals such as alkaloids, flavonoids, phenols etc. The quantitative analysis of phenols, flavonoids, alkaloids was performed by spectrophotometrically. The evaluation of anti-oxidant activity was determined by DPPH and ABTS methods. The anti-diabetic activity of crude extracts was determined by in vitro α-amylase inhibition method. The methanolic extract was significantly showed DPPH IC50 inhibition at 20 µg/mL, ABTS inhibition IC50 at 30µg/mL and in vitro α-amylase inhibition IC50 at 50µg/mL. It is concluded that methanolic extract has the potential source of anti-oxidant and anti-diabetic activity.

Keywords: Jurinea Dolomiaea Boiss, Antioxidant, Anti-diabetic, DPPH, ABTS, Brassica oleraceae, In Vitro α-Amylase Inhibition.

DOI: 10.7176/CMR/15-1-04

Publication date:May 31^st 2023