In Vitro Micropropagation of Trichopus Zeylanicus Gaetn. Through Seed Explant

Abstract

Trichopus zeylanicus Gaertn. belonging to family Trichopodaceae is a rare herb with extraordinary medicinal properties found in the Agasthyar hills in Thiruvananthapuram of Kerala.  Trichopus zeylanicus Gaertn.  was regenerated in vitro on MS medium supplemented with different concentrations and combination of auxins and cytokinins. The effect of phytohormones on in vitro establishment of explants, production of multiple shoots and rooting of established shoots were studied. MS medium supplemented with 4.44 mM BAP produced maximum of 32 multiple shoots from seed explant. MS medium supplemented with 2.46 mM IBA induced maximum rooting. The rooted plantlets were acclimatized with a survival rate of 75 per cent to 80 per cent.

Key words: Micropropagation, Trichopus zeylanicus Gaertn., Multiple shoots, Seeds.