Non-Phenolic Compounds from the Stem Bark of Red Mangrove (Rhizophora stylosa) and Evaluation of Their Cytotoxicity and Larvacidal Activity

Abstract

Non-phenolic compounds namely lupeol and ?-sitosterol had been separated from the stem bark of Indonesian red mangrove (Rhizophora stylosa). The lupeol obtained a colorless powder with a melting point of 210-212 ^oC, whereas ?-sitosterol obtained as a colorless powder with a melting point of 136-137 ° C.  Isolates were separated using chromatographic techniques, purified by recrystallization, and their structures determined by spectroscopic methods (UV, IR, and MS) and compared with literature data. They showed cytotoxicity against Artemia salina and  larvacidal acitivity against Aedes aegypti larvae.

Keywords: Rhizophora stylosa, lupeol, ?-sitosterol, Artemia salina, Aedes aegypti