Quantitative and Qualitative Phytochemicals Analysis of Muntingia calabura

Abstract

Muntingia calabura is widely cultivated and has become one of the common roadside trees in Indonesia. The present study investigates the qualitative and quantitative analysis of the major bioactive constituents of plant Muntingia calabura in some solvents (water, methanol, ethanol, chloroform, ether, and citrid acid). Phytochemical screening method was used to identify qualitative analysis of bioactive component. While the quantitative analysis was analysed by UHLPC. The results showed the highest percentage extract concentration was resulted by methanol followed by water, ethanol, chloroform, ether and citric acid 13.15%, 11.93%, 9.57%, 9.04%, 4.52% and 3.69% respectively. The bioactive component ie., saponins, flavonoids and tannin were found in all those solvent. The higher concentration of flavonoid, saponin and tannin were resulted from polar solvent ie., water, methanol and ethanol. While non polar solvent has lower in concentration. Epigallocatechin Gallate (EGCG) and Genistein content of extract Muntingia calabura from methanol solvent were 135.15  µg/g  and 136.29 µg/g respectively.

Keywords: Phytochemical constituents, Muntingia calabura, qualitative and quantitative analysis.