The Role of Sarang Semut (Myrmecodia pendans) Flavonoid’s Fraction in Proliferation and Angiogenesis Inhibition of Human Tongue Squamous Cell Carcinoma

Harun Achmad, M. Hendra Chandha, Sri Ramadhany, Hendrastuti Handayani, Rasmidar Samad


Background: Squamous cell carcinoma is the most common type of cancer that occurs in the oral cavity which is about 90-95 % of total malignancy in the oral cavity. The aim of this study was to analyze the effect of the flavonoid’s fraction of Sarang Semut (Myrmecodia pendans) as an anti-cancer to angiogenesis inhibition by suppressing the VEGF and IL-8 expression in SP-C1 human tongue cancer cell. Material and Method: This research was conducted with the pure laboratory experimental method using Supri’s-Clone 1 (SP-C1) human tongue cancer cell culture. This research was started with cytotoxicity test to obtain the fraction of flavonoid that possesses anti-cancer potentiality and angiogenesis inhibition test. Result: The result of this study showed that ethyl acetate and ethanol fraction of flavonoid had a role in inhibiting the VEGF protein and interleukin 8 expressions in SP-C1 tongue cancer cell. ANOVA test with significance level (? = 0,05) which resulted in highly significant difference in the concentration with a mean optical density (OD) absorbance ethanol fraction (p = 0,00), ethyl acetate fraction (p = 0,00), and controls (p = 0,00) for the suppression of VEGF protein expression and interleukin-8 of SP-C1 human tongue cancer cell. Western blotting analysis showed that ethyl acetate fraction of flavonoid application to SP-C1 human tongue cancer cell showed a decrease in SP-C1 protein expression.   Conclusion: Flavonoid fraction of sarang semut inhibit the angiogenesis in SP-C1 tongue cell cancer by suppressing the VEGF and interleukin 8 protein expression. Ethyl acetate fraction from sarang semut’s flavonoid inhibited the expression signal transduction factor from SP-C1 tongue cancer cell.

Keywords: SP-C1 tongue squamous cell carcinoma, sarang semut flavonoid’s fraction, angiogenesis, VEGF, interleukin-8.

Full Text: PDF
Download the IISTE publication guideline!

To list your conference here. Please contact the administrator of this platform.

Paper submission email:

ISSN (Paper)2224-3208 ISSN (Online)2225-093X

Please add our address "" into your email contact list.

This journal follows ISO 9001 management standard and licensed under a Creative Commons Attribution 3.0 License.

Copyright ©