Evaluation of Biocontrol Agents and Chemical Inducers for Managing Vascular Wilt of Tomato Caused by Fusarium oxysporum f.sp. lycopersici

Abstract

The study was conducted to evaluate the efficiency of biocontrol agents (Bacillus mycoides and Trichoderma viride) and the chemical compounds, ASM and Preservepro against Fusarium oxysporum f.sp. lycopersci the causal agent of tomato wilting disease in culture media and under greenhouse conditions .The results showed that all the control agents exhibited an inhibition rate on F. oxysporum growth on both culture media and under natural conditions .The addition of bio and chemical agents to the contaminated soil induced significant reduction in disease incidence and disease severity associated with increase plant dry weight .The disease incidence, severity and plant dry weights in T.viride, B.mycoides, ASM and Preservpro were found, 26.70,20.00,53.30,13.30% and 13.30,13.30,33.30,6.70% and 4.43,4.80,3.56 ,5.30 g/plants respectively compared with 86.70% ,70.00,and 0.60 g/plant in control treatment .Additional reduction in disease incidence ,disease severity and increase in plant dry weights were obtained on plant grown in soil treated with combination of control agents .The disease incidence , severity and plant dry weights were found in the combination of T.viride + B.mycoides, ASM +T.viride , ASM + B.mycoides, Preservepro + T.viride , Preservepro+B.mycoides, ASM + Preservepro 13.30, 26.70, 21.70, 8.30, 5.00, 13.30% and 6.70, 10.30, 13.30, 0.00, 0.00, 3.33% and 5.00, 4.00, 4.50, 5.80, 6.03, 5.40 g/plant respectively compared with 70%, 86.70% and 0.6 g/plant in control treatment.

Keywords:Fusarium oxysporum, Bacillus mycoides, Trichoderma viride, ASM, Preservpro, induce resistance.