IN VITRO Proliferation of Plantain using Different Concentration of Auxin and Cytokinin

Abstract

This study was carried out in the Tissue Culture Laboratory of Nigerian Agricultural Quarantine Service (NAQS), moor plantation, Ibadan. The explants used were obtained from National Horticultural Research Institute (NIHORT) and the growth parameter studied is the number of proliferated buds. MS (Murashige and Skoog) media supplemented with 0.18mgl^-1NAA + 2.3mgl^-1BAP supported the growth and bud formation in meristem culture of Musa paradisiaca cv. Agbagba during the initiation stage. After the first apical buds appeared, the buds were transferred to MS media supplemented with different combinations of Naphthalene acetic acid (NAA) and Benzylaminopurine (BAP) at different concentrations (0.0mgl^-1NAA + 0.0mgl^-1BAP, 0.1mgl^-1 NAA + 3.5mgl^-1 BAP, 0.18mgl^-1 NAA + 4.5mgl^-1 BAP, 0.26mgl^-1 NAA + 5.5mgl^-1 BAP). The results obtained showed that proliferation media supplemented with 0.18mgl^-1 NAA + 4.5mgl^-1 BAP yielded the highest number of buds. This study however revealed the effect of hormone in the initiation and proliferation of plantain buds as essential and MS medium with NAA (0.18mgl^-1) and BAP (4.5mgl^-1) was the optimum concentration required for the proliferation of plantain.

Keywords: Optimum concentration, In-vitro, MS medium, proliferated buds