Effects of Topical Application of Green Apple Extract on Excisional Wound Healing in Female Rabbits

Abstract

In the present study wound healing efficiency of green apple alcoholic extract of the whole fruit in the form of ointment , using one type wound model ( full thickness incisional  wound )was evaluated in twelve female rabbits. ethanol solvent was used to prepare the extract. chemical analysis of  green apple alcoholic extract   reveals  the presence of alkaloid, saponin ,tannin in Flavonoid, Glycosides, polysaccharides , proteins, Triterpens  and Carbohydrates. tow Circular wounds were inflicted on the animals which ,divided into three groups , four animals in each group. the ointment of green apple was applied topically with 5mg twice daily. Wound area was measured  and the percentage of wound reduction ( wound contraction rate) per a day was calculated. The effects were studied on excissional wound were hyperemia , exudation ,   were percent of wound contraction and length of wound macroscopically, and infiltration of inflammatory cell (macrophage ,neutrophils),granulation tissue formation  microscopically. Treated wounds induced complete wound healing in shortest period (11 days) while control wounds show non complete  healing till the end of experiment ( 14 days).

Keywords: rabbits, wound healing, green apple