Detection of Equine Herpesvirus Infection: Sensitivity Assay of Polymerase Chain Reaction

Abstract

The study was designed to identify the potential diagnostic tool with minimum detection limit in a clinical sample for Equine Herpesvirus Strain -1. An approach of molecular diagnostics was used using reported primers of polymerase chain reaction using in the sample obtained from repository. The PCR primers were specific to ORF 16 gene of EHV-1. Sensitivity assay of PCR detection was performed by making dilutions of EHV-1 positive DNA sample and running each dilution in a PCR and visualizing amplicons in ethidium bromide stained agarose gel under UV radiation. Study was valuable in determining the efficiency of PCR for quick and reliable source as disease survilance.disease surveillance is as equal important as treatment, Without surveillance and diagnostics it is not possible to prevent and control any disease.

Key words: EHV-1, Equine Herpes Virus; PCR, Polymerase Chain reaction, ORF 16.