Comparative Study between using of Biomass and Extract of Trichoderma Viride to Inducing Anticancer (TIA) Vincristine Production from Catharanthus Roseus in Vitro

Abstract

This experiment was conducted in college of Agriculture , Al-Qasem Green university in 2015 ,to study the different of effects between using Trichoderma viride as biomass and as extract to induce the production of anticancer vincristine in vitro  using MS medium supplemente with (2,4-D) and (BA) in (1+1) mg /L concentration to induce callus  production then inoculated the callus directly with biomass of T.viride by single spore .In first method and adding extract of T.viride to the MS medium in the second method .The study include quantity and quality determination of vincristine by HPLC technique .Results revealed that the highest values was for the using of T.viride as biomass which gave(25.120)µg/g vincristine and the lowest value was for T.viride as extract which gave(15.346)µg/g vincristine .also results explained that adding of T.viride as biomass and the extract cause decrease in the fresh and dry weight of callus inducing from leaves of C.roseus and the highest decrease was to biomass method (200, 0.019) mg followed by  extractmethod(340,0.031)mgrespectively.

Keywords: Trichoderma viride in vitro, vincristine extract.