Aqueous Extracts of Anogeissus Leiocarpus (DC.) Guill. & Perr. and Terminalia Glaucescens Planch ex Benth. Inhibited Helicobacter Pylori

Abstract

The inhibitory effects of methanol, dichloromethane and aqueous extracts of Anogeissus leiocarpus (DC.) Guill. & Perr. and Terminalia glaucescens Planch ex Benth. (family Combretaceae) reportedly used in the treatment of gastrointestinal diseases including stomach disorder and ulcer are investigated to ascertain and justify their use in traditional medicine. Nineteen (19) strains of Helicobacter pylori including 18 clinical isolates and Helicobacter pylori ATCC 43504 were used in this study. The susceptibility and minimum inhibitory concentration (MIC) testing were performed using the agar dilution procedure guidelines of the Clinical and Laboratory Standards Institute. bactericidal studies were performed using viable counting techniques. The MIC and MBC values for the susceptible strains ranged from 0.08 mg/mL to 1.25 mg/mL and 0.16 mg/mL - 2.5 mg/mL respectively Bactericidal study revealed a dose- and time-dependent decline in surviving population of H. pylori AB005 at concentrations equivalent to MIC, 2 x MIC and 4 x MIC (Figures 1-4). The aqueous extracts of Anogeissus leiocarpus stem bark and root gave a 100% kill at 6-hour exposure time with concentration equivalent to 4x MIC (i.e. 0.32mg/mL). The antimicrobial activities demonstrated by extracts of the plants on Helicobacter pylori revealed the presence of therapeutically potent antibacterial compounds and thus justify the use of these medicinal plants for the treatment of peptic ulcer disease.

Keywords: Anogeissus leiocarpus (DC.) Guill. & Perr.; Terminalia glaucescens Planch ex Benth.; Helicobacter pylori; peptic ulcer disease; bactericidal activity