Molecular study in some species of family Papaveracea and Fumariaceae in Iraq and Iran by used matK gene and ITS4/5

Abstract

The position between the three families Papaveraceae, Fumariaceae and Hypecoaceae is one of the most problematic taxonomic. To understand the phylogeny relationship of these genera thirty six samples of Papaveraceae s.l. were extracted for DNA analysis. Three primers based on ITS of nrDNA ITS3,5 ITS4,5 and matk chloroplast gene were used in PCR amplification. Phylogenetic relationships among genera and species of Papaveraceae s.l. based on Neighbor-joining (NJ), UPGMA and ML-DTR-GL 1000 models were drowning. The clades of phylogenetic relationship clearly support relationships of Fumariaceae to the Fumarioideae in the family Papaveraceae s.l.

Keywords: Papaveraceae, Fumariaceae, classification, ITS, matk