In Vitro Mass Propagation of Enicostemma Littorale Blume from Shoot Tip Explants

Abstract

In vitro rapid regeneration and mass propagation was deliberated from shoot tip explants of Enicostemma littorale Blume. The explants were cultured on MS (Murashige and Skoog, 1962) medium containing various concentration of cytokinins ranging from 5 µM  to 25 µM. When compared to KIN, BAP was found to respond well in shoot multiplication and number of shoots.  Large number of shoots was produced from all concentration of BAP and KIN. Maximum number of shoots and highest frequency of 100% shoot induction was observed on MS medium containing 15 µM KIN and BAP.  The excised shoots were then transferred to MS medium augmented with IBA and NAA in various concentration for root induction.  The roots were initiated and well developed in 2µM of both the auxins.  The in vitro raised plantlets were successfully transferred to soil through hardening and acclimatization.

Key words : Enicostemma littorale, cytokinins, auxins

Abbreviations : BAP - 6-benzylaminopurine; KIN - kinetin (6-furfuralaminopurine; IBA-indole butyric acid; NAA - Naphthalene acetic acid