The Number of Lymphocyte and Monosit due to Hypoxic Hypoxia

Abstract

Oxygen have an important role in body metabolism.  Lack of oxygen can caused uncomplete metabolism. There will be increasing consumtion of glucose in anaerob metabolism. Hypoxia can stimulate the circulatory system and hematology to improve oxygenation function. This research purpose to knows effect of hypoxic hypoxia duration to the lymphocytes and monocytes on Rattus norvegicus. This is experimental research, pre and post control group design. Hypoxia condition made from modification of hypoxia chamber with 10% oxygen level from total volume of chamber. Subject is male of Rattus norvegicus 2 month’s old weight 150 – 200 grams. Measuring of lymphocytes and monocytes used manual hemocytometer and the hemoglobin use spektrofotometer. Data analysis used t-test and oneway anova CI 95 %. Results of analysis with Oneway-ANOVA statistical test showed there was no difference in the number of lymphocytes were statistically significant in the fourth study group after treatment (p = 0.31). Similarly Oneway-ANOVA statistical test showed there was no difference in the number of lymphocytes were statistically significant in all four study group after treatment (p = 0.99). The number of lymphocytes and monocytes increased due to hypoxic hypoxia although not statistically significant. Increasing the number of lymphocytes highest in the group induced hypoxic hypoxia 36 hours and the number of monocytes highest in the group induced hypoxic hypoxia 12 hours.

Keywords : lymphocytes, monocytes, hypoxia, oxygen