Vitamin D Level in Patients with Bone Diseases and Dental Caries

Abstract

VITAMIN D, it is a steroid (steroids are group of substances derived from perhydrocyclopentano phenanthrene nucleus) prohormone with antirachitic properties, also known as cholecalciferol, calciferol. calcipotriol ,ergocalciferol, and vitamin D2. HPLC is a new come in the field of liquid chromatography. It is probably the single most used analytical technique today. HPLC system is unique among laboratory instruments because it can be assembled using components from different manufacturers and suppliers. A wide variety of tissues and cells, including the brain, gonads, breast, skin, mononuclear cells, and activated B and T lymphocytes, possess VDR. The first insight into biological action of (1,25 (OH)2 D) in non calcemic tissues was the observation that promyelocytic leukemic cells with a VDR transformed into mature, biochemical functioning macrophages after being treated with (1 , 25 (OH)2 D)(35) During 5 months (March, April, May, June and , August) 60 blood samples (29 males and 31 females ) were collected from healthy volunteers and simultaneously 92 blood samples (33 males and 59 females ) were collected from patients attending to AL – Hakims General Hospital, AL- Zahra Hospital , and some other private clinic in AL- Najaf city. All of the patients are from Al- Najaf city, Iraqi people, they lived in this society with poor to middle life style and the income of these families approximately at the same.The study primarily was intended to adapt a method for measuring vitamin D levels in serum samples in order to make reliable evaluation of this vitamin in Iraqi population (Al-Najaf governorate). The HPLC method was the method of choice in this field .The reverse phase HPLC method has a very high sensitivity and needs only a very small amount of sample without any complicated pretreatment for the used serum which may be a major source of errors. The mean of vitamin D levels in both sexes and in different seasons was 19.21ng / ml the other related statistical parameters and serum calcium level.

Keywords: Vitamin D, Calcium, HPLC, Bone disease, Rickets, Seasonal change, Osteomalasia, Osteoporosis, Dental caries, Postmenopausal.