In-Vitro Antibacterial Activity of Ruta Chalepensis (Tenadam) and Justicia Shimperiana(Senel) Plants against Some Bacterial Human Pathogens

Habtamu Tedila


The leave extracts of Ruta chalepensis and Justicia schimperiana were the most powerful medicinal value. The plant extraction followed by ethanol, methanol acetone, diethyl ether and hexane by using disc diffusion and broth dilution methods (MIC) against six human pathogenic bacterial strains (Shigella dysentery, Escherichia coli, Streptococcus aeruginosa, Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella typhi and Klebsiella pneumonia). The methanol extract of J. schimperiana showed strong inhibition activity against S. dysentery and E. coli with a zone size of 14.5±0.5mm and 16±0.2mm and MIC values of 3.12mg/ml against E. coli and S. dysentry. Highly prominent activity was produced by the ethanol extract of R. chalepensis with the highest zone of 15±0.5mm diameter observed in S. typhi, followed by S. aureus13±0.11mm with the MIC value of 1.56mg/ml against S. typhi. Four antibiotics were used as standard for the testing of antibacterial activity against six different human pathogens. Among the antibiotics Ciprofloxin showed maximum zone of inhibition ranging from 20-35mm followed by Kanamycin, Tetracycline and Chlormphenicol.

Keywords: Antibacterial activity, Broth dilution, Disc diffusion, Human pathogens

DOI: 10.7176/JHMN/63-03

Publication date:June 30th 2019

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