Presence of Class I and Class II Integrons in Methicilin Resistant Staphylococci and Their Relations with Antibiotic Resistance: A Preliminary Study from Turkey

Abstract

Background: Emergence of antibiotic resistance is a major public health concern. It is known that antibiotic resistance is transferred by different ways. Integrons as one of these mechanisims cause to spread antibiotic resistance in Gram negative bacteria but also it is shown to be effective for transferring genes in Gram positive bacteria. In the present study we aimed to examine the prevalence of class I and class II integrons in MRSA and MRCNS strains isolated from patients and to determine the relationship between antibiotic resistance and the presence of integrons. Methods: Sixty four MRSA and 62 MRCNS strains were included in this study. Antibiotic susceptibility testings were performed. Genomic and plasmid DNAs were extracted and Polymerase Chain Reaction (PCR) was used for the detection of the intI and intII genes. The PCR products were visualised in 1.5 % agarose gel electrophoresis. Pearson chi-square test and Fisher’s exact test were used for comparing categorical variables. Results: Among 126 staphylococci 11 (8.7% - 4 MRSA and 7 MRCNS) were shown to carry class I integron; whereas 7 MRCNS (5.5%) were class II positive. Both of class I and class II integrons were detected to possess in four MRSA (3.2%). There was no statistically significant relation between presence of integrons and resistance to each of antibiotics (p>0.05). Conclusion & Recommendation: In the present study we did not find any significant relation between resistance rates and the presence of integrons but we suggest that these results showed an important data about the extended distributions of integrons not only among Gram negative bacteria but also in staphylococci.

Keywords: MRSA, MRCNS, class I integron, class II integron, anbiotic resistance

DOI: 10.7176/JHMN/75-07