Detection of High-Risk Human Papillomavirus by Chromogenic in Situ Hybridization Method in Liquid-Based Cervicovaginal Cytology Specimens with Atypical Squamous Cells of Undetermined Significance

Abstract

Background: Cervical cancer is the second most common cancer after breast cancer in women worldwide. Owing to comprehensive cervical screening programs, a significant decrease has been observed in the incidence of invasive cervical cancers. Human Papillomavirus (HPV) is the underlying etiology in 99.7% of the cases of cervical cancer and is a major risk factor for the development of precancerous and cancerous cervical lesions. Chromogenic in situ hybridization method (CISH) is one of the methods used to detect high risk HPV in liquid -based smears. Aims: In this study, we conducted an archive search in the department of pathology and we used chromogenic in situ hybridization technique to investigate whether HPV DNA was present in liquid -based smears  of 68 patients who had been  found to have atypical squamous cells of undetermined significance (ASCUS) and whether a cervical intraepithelial lesion was present in the biopsy samples of patients who were positive for HPV DNA.Methods and Material:. We prepared additional samples from liquid -based preparations obtained by the ThinPrep method. The CISH procedure was performed on the Benchmark Automated Slide Stainer (Ventana) according to the manufacturer’s standard protocol.Results and Conclusions: We found HPV DNA in  the samples of 10 patients by using INFORM HPV III high risk (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 68, and 70) (Ventana, Tucson, AZ) probe, with CISH method on each additional sample, and biopsy  results of the respective  patients, indicated  low grade squamous intraepithelial lesion (LGSIL) in 8 patients and high grade squamous intraepithelial lesion (HGSIL) in 2 cases.

Keywords: Human papillomavirus, chromogenic in situ hybridization, atypical squamous cells of undetermined significance

DOI: 10.7176/JHMN/80-10

Publication date:September 30^th 2020