Fluorescence Microscope: A Review Article

Abstract

A fluorescence microscope is an optical microscope that uses fluorescence and phosphorescence to study properties of organic or inorganic substances. Therefore, the objective of this seminar paper is to understand the principles of fluorescence microscope and acquire knowledge about fluorescence microscope. The basic task of the fluorescence microscope is to let excitation light radiate the specimen and then sort out the much weaker emitted light to make up the image. Fluorescence microscopy is a rapid expanding technique, both in the medical and biological sciences. Therefore, laboratory practitioners and other basic science professions should know in details the principles and application of fluorescence microscope. The environment of the fluorochrome stained specimen from fading should be protected.

Keywords: Fluorescence Microscope, Fluorochrome, Image and Specimen