Detection of ERG11-2 gene in candida spp. wich resistant to some antifungal agents by Real Time –PCR

Abstract

The present study was aimed to determine ERG11-2 that responsible of resistance to some antifungal agents in candida spp. Atotal of( 107) samples were obtained  fromdifferent clinical  cases included(36) oral swabs from children suffering from oral thrush  , (32) vaginal swabs from women suffering from vaginal candidiasis  ,(39) urine samples from individuals suffering from urinary tract infection) and( 25) samples from sewage water as a control samples in the molecular study .The result of morphological and biochemical tests showed For pathogenic samples that 83(77.5%) isolate of candida spp. Included 36/31(86.1%) from oral samples,32/28 (75%) from vaginal swabs, 28/39 isolates (71.7%) from urine samples while the result of non- pathogenic samples revealed that 20 (80%) isolate of candida spp. Detecting of,ERG11-2 resistant gene was done by RealTime-PCR technique . Results showed that all isolates 31  (100%) were carrying ERG11-2 that responsibe of resistance  in candida to some antifungal , Did not record any isolated from control samples from sewage water wich amounted to (9) the ERG11-2resistantgene

Key words: ERG11gene , candida sp., RT-PCR