Antibacterial and Antioxidant Activities of Various Fraction of Leea rubra (Leeaceae)

Abstract

The present study was designed to evaluate the antibacterial and antioxidant activities of hexane, ethylacetate and ethanol extracts of Leea rubra (Leeaceae) roots and stems, which has been used as a Lanna Traditional Medicines for Màhòog. Each extract was tested for antibacterial activity by agar diffusion method and microbroth dilution method and antioxidant activity by 2,2´-azino-bis(3-ethyl- benzthiazoline-6-sulfonic acid) (ABTS) free radical scavenging assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay and ferric reducing antioxidant power (FRAP) assay. The ethylacetate extract of L. rubra root showed the highest antibacterial activity against gram-positive (IZD=15.5.0±0.5 to 17.5±0.5 mm, MIC=0.098-1.562 mg/ml).  While the ethanolic extract of root showed the strongest antioxidant activity in ABTS, DPPH and FRAP method (TEAC=0.888±0.001, 0.849±0.020 and 0.733±0.037, respectively). The data obtained from this study confirms the traditional use of L. rubra for treatment Màhòog.

Keywords: Leea rubra, antioxidant activity, antibacterial activity