PCR Detection of Putative Hemolysin and Aerolysin Genes in An Aeromonas Hydrophila Isolates from Diarrhea in Babylon Province

Abstract

Aeromonas spp are considered as apportunistic infection and enterotoxgenic pathogen and can cause severe diarrhea . A total  of one hundred and forty two stool samples were collected from patients with diarrhea at Babylon hospital for maternity and pediatrics of a period  between December 2012 to June 2013.The 6(4.22%  )strains of Aeromonas hydrophila were isolated from 142samples of diarrhea stools .The Aeromonas  hydrophila identified based on colony and microscopic morphology ,biochemical tests and confirmed by Api 20E .Then investigation was done on  hemolysin ,All the isolates of Aeromonas hydrophila have the ?- hemolytic activity on the blood agar .the polymerase chain reactions carried out to detect the presences of hemolysin and aerolysin genes in 6 Aeromonas hydrophila isolates .A100%(6 isolates)of  Aeromonas hydrophila isolates were contained hemolysin genes (ahh 1a)and 50%(3 isolates)  were contain aerolysin gene (aerA).the band appearance in amplified gene bacteria shows molecular weight of hemolysin (130  p) and the molecular weight of aerolysin ( 309  bp) .

Key word:Aeromonas, aerolysin, hemolysin, PCR