Using of Plant Extracts for Cinnamon, Syzygium and Thyme to Degradation of Aflatoxin B1

Abstract

This study had done in post graduate studies lab in Dep. OF Biology- College of Science – Diyala University, plant extracts of Cinnamon (Cinnamomum zylanicum) ,Syzygium (Syzygium aromaticum) and Thyme (Thymus vulgaris) were used with four different extraction methods to show their activities in degradation of aflatoxin B1. The results proved that plant extracts were effecting on AFB1 ,and showed that all extraction methods were in the same activity on AFB1 at 24 µg\kg concentration .Hexanic extract for Thyme at 250 µg\ml  concentration gave's the highest degradation percentage of AFB1 98.7% ,followed by alchoholic extract for Syzygium at 500 µg\ml concentration with degradation percentage for toxin 97.5%,  cold water extract for  Cinnamon at 1200 µg\ml concentration with degradation percentage for toxin 96.9% , alchoholic extract for Thyme at 1000 µg\ml concentration gave's degradation percentage for toxin 93.7%, cold water extract for Thyme at 850 µg\ml concentration with degradation percentage for toxin 84.3%, and the other extracts like hot water extract for Thyme at 1350 µg\ml ,alchoholic extract for Cinnamon at 180 µg\ml, cold water extract for  Cinnamon at1250 µg\ml, hot water extract for Syzygium at 1000 µg\ml and hexanic extract for Cinnamon at 110 µg\ml all of them gave degradation percentage 78.7% on AFB1 concentration .So hot water extract for Syzygium at 1000 µg\ml  and hexanic extract for Cinnamon at 110 µg\ml concentrations lowered the percentage of AFB1 in 78.1%, and hot water extracts for Thymus at 1250 µg\ml and Syzygium at 1100 µg\ml concentrations lead to lower AFB1 concentration with 77.5%  , Hexanic extract for Syzygium at 200 µg\ml and cold water extract for it at 850 µg\ml concentrations led to destroy AFB1 with 75% , The cold water extract for Thymus at 1250 µg\ml and hexanic extract for Syzygium at 250 µg\ml concentrations led to AFB1 degradation with 72.5%.Then both extractions for Cinnamon the hexanic at 100 µg\ml and hot water at 1300 µg\ml  concentrations led to AFB1 degradation with 62.5% ,53.75% on respectively , Less degradation AFB1 percentage 12.5% from alchoholic extract for Syzygium at 400 µg\ml . other plant extracts such as hexanic extract for Thymus at 200 µg\ml, cold water extract for Syzygium at 750 µg\ml, alchoholic extract for Cinnamon at 220 µg\ml concentration were not affected by AFB1 concentration.

Keywords: Plant extracts, Aspergillus flavus fungus and Aflatoxin B1.