Anti-Bacterial Activity of the Prophet Mohammad (SWS) Drink's against Pathogenic Bacteria

Abstract

Drink of the Prophet Mohammad (Kefiran) which is acidifying fermented milk is accepted as a good example of a probiotic mixture of bacteria and yeast. In this study colorimetric VITEK-2 Compact system was used to identify isolates and to detect susceptibility test to several antimicrobial agents. The study also investigated the antimicrobial activity of 24, 48, 72 and 96 hours fermented DPM (kefiran) against isolated bacteria from UTI patients. The bacteria isolated were eleven gram negative bacteria, included, Acinetobacter baumannii 8 (8%), Enterobacter cloacae 4 (4%), Escherichia coli 16 (15%), Klebsiella oxytoca 6 (6%), Klebsiella pneumonia 11 (10%), Micrococcus luteus 3 (3%), Morganella morganii 4 (4%), Proteus mirabilis 7 (7%), Proteus vulgaris 4 (4%), Pseudomonas aeruginosa 12 (11%) and Serratia marcescens 4 (4%) and four gram positive bacteria, Enterococcus cloacae 2 (2%), Enterococcus faecalis 2 (2%), Staphylococcus aureus 15 (14%), and Staphylococcus haemolyticus 6 (6%). The results of antimicrobial susceptibility test against gram negative and positive bacteria showed the majority of isolates were resistant to most antimicrobials. The MIC values ranged from (? 0.125 - ? 32 ?g/ml). The inhibition zone of 24 hours incubation of DPM (Kefiran) against all isolates was between (7-8 mm).The effects at 48hours of incubation of DPM (kefiran) against all isolates was between (8-9 mm). The effects at 72 hours of incubation the inhibition zone was between (10-11 mm). The maximum activity of the Prophet Mohammad Drink's was recorded at 96 hours of incubation period against all isolates; the inhibition zone was between (10-12 mm).

Keywords: Urinary tract infections, Vitek-2 compact, Drink of the Prophet Mohammad (Kefiran), antimicrobial activity, pathogenic bacteria.