Effects of Temperature, pH And Some Monoatomic Sulphur Compounds on Rhodanese from Sheep Liver

Abstract

The ubiquitous enzyme rhodanese (cyanide: thiosulphate sulphur transferase E.C.2.8.1.1.) in sheep liver was studied. The effects of temperature, pH and some monoatomic sulphur compound were investigated. Results revealed that the temperature and pH optima were 30^OC and 8.5 respectively. Cysteine, dithio oxiamide, glutathione and 2-mercaptoethanol were not able to substitute for thiocyanate in rhodanese assay; these monoatomic sulphur compounds never served as sulphur donors in sheep liver rhodanese catalyzed reaction of cyanide detoxification.