Phylogenetic Tree Analysis of Dermatophytes using Sequence of the 18S rRNA Gene ITS Region

Abstract

Dermatophytes usually identified on the basis of   isolation patterns together with conidial morphology, and some-times with physiological characters, such as the hair perforation and urease tests. In some cases, morphological identification can be difficult because there is great variation and polymorphism among isolates of the same species. Recent, genotyping approaches have proven to be useful for solving problems of dermatophyte classification. Molecular identification such as ITS sequencing could become part of the diagnostic for dermatophytosis, In present study, results show genetic analysis of the tree shows the evolutionary relationships between species, which helps in the diagnosis of suspected species, especially T.rubrum and M.canis .

Keywords: phylogenetic tree, T.rubrum , M.canis , PCR, Dermatophytes.