A Study of the Phytochemical Properties and Synergistic Action of Leaf Extracts of Dodonea Viscosa Linn, Annona Comosus (Linn) Merr Peel and Citrus Senensis Peel on Aeromonas hydrophila and Salmonella Species
Abstract
The leaf of Dodonea viscosa Linn, peels of Annona comosus (Linn) Merr and Citrus senensis are widely used traditional remedies against various ailments, such as digestive system disorders like: indigestion, ulcers, diarrhoea, constipation, upset stomach and tonic to digestive system. The major chemical constituents reported from the plant parts are alkaloids, flavoniods, saponins, tannins, steroids, triterpenoids and phytosterols which show that these plant parts can be a potential candidate to be use as a therapeutic agent. The aim of the study was to determine the synergism at a concentration ratio of 1:1 between the three extracts using discs diffusion, broth tube dilution and fractional inhibitory concentration techniques against six Salmonella paratyphi B, one Salmonella typhi and three A. hydrophila. In vitro anti-salmonellae and A. hydrophila activities of the extracts were confirmed and no synergism was demonstrated (P = 0.05).
Keywords: Bioactivity, phytochemicals, synergism, Dodonea viscosa, Annona comosus, Citrus senensis, MIC, MBC, FIC.
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ISSN (Paper)2224-3186 ISSN (Online)2225-0921
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