Molecular Analysis of Prolactin Receptor Gene of Hyperprolactemic Iraqi Patients

Abstract

Prolactin hormone is a polypeptide hormone of pituitary origin, whose production is controlled by dopamine. Hyperprolactinemia is characterized by increased production of     prolactin hormone levels often leading to reproductive dysfunction. The aim of this study is to investigate if there is any genetic variation or changes in nucleotide sequences of Exon 1 of prolactin receptor gene behind infertile cases of Iraqi women patients suffering from hyperprolactinemia. For this study 15 samples from infertile hyperprolactinemic women were taken and genomic DNA was extracted, specific primers was designed to amplify specific portion of prolactin receptor gene (Exon 1) using PCR technique . The PCR products of both healthy and hyperprolactinemic infertile women were subjected for DNA sequencing by machine AB137 30KL, Applied Biosystem, Macrogene company, USA. Polymorphic variants were identified , data obtained which is heterozygous SNP and SNPs was found between individuals. The DNA sequencing results of receptor from patients was found to be compatible 99% and score 481 with the wild type sequence of gene bank. And the differences may be attributed to one transition mutation (G/A) at position 61 of exon 1. Its substitution mutation that leads to changes amino acid from Arginen  to Lysin.

Keywords:Prolactin receptor gene, Hyperprolactinemia, Mutation