Isolation and Identification of Alkaloidic Extract of Capparis spinosaL Buds and Study of Its Cytoxicity and Antibacterial Activity.

Abstract

This study was carried out for isolation and identification of alkaloids from Capparis spinosa L. buds, by using several solvents, qualitative reagents and gas chromatography-mass spectrum (GC – mass) technique. The isolated alkaloidic extract was found to contain many active chemical compounds. Antibacterial activity of alkaloidic extract was investigated against some pathogenic bacteria which represented by Escherichia coli and Staphylococcus aureus.

The alkaloidic extract concentrations (125 , 250 and 300 mg/ml) recorded inhibition zone diameters equal to (16 , 19 and 19 mm) against E. coli bacteria and (16 , 20 and 20 mm) against S. aureus bacteria. The concentration of (250 mg/ml) was found to be the maximal inhibitory concentration against both pathogenic bacteria. It is recommended to use alkaloids of Capparis spinosa to treat different diseases caused by these bacteria instead of antibiotics but this demands more clinical and pharmaceutical studies.

Keywords: Capparis spinosa buds, Alkaloids Extract, Pathogenic Bacteria, GC-mass technique, Maximal inhibitory concentration.