Enterotoxigenicity Profile of Salmonella Enterica Serovar Typhimurium in Suckling Albino Mice

Abstract

This study was carried out to investigate the enterotoxigenic potentials of Salmonella enterica serovar Typhimurium from different food samples in suckling albino mice. A total of 27 food samples comprising sample A (9), sample B(9) and Sample C(9) were collected randomly from different markets in Uli community and screened for the presence of Salmonella enterica serovar Typhimurium using pour plate technique. The isolates obtained were characterized and identified using their colonial descriptions, morphological and biochemical characteristics. Intragastric method in suckling mice was used to evaluate the enterotoxigenicity potententials of the isolates using gut/body ratio (G/B) and fluid accumulation ratio (FAR). Salmonella enterica serovar Typhimurium (ST1,ST2,ST3,ST4,ST5,ST6) was detected in 14(51.85) food samples out of 27 food samples screened, of which the isolate was discovered mostly in sample B. ST5 was significant (P<0.05) for enterotoxin after 4 h exposure and ST2, ST3, ST5 and ST6 were significant (P<0.05) after 24 h. It was observed that most of the strains of Salmonella enterica serovar Typhimurium tested, were positive to enterotoxin mainly after 24 h, of which ST5 proved to be most enterotoxigenic after 4 h and 24 h.