Optimum Conditions for Reducing Sugar Production from Eichhornia Crassipes Biomass Using Trichoderma Virdae

Abstract

Evaluation of optimum conditions for reducing sugar production from Eichhornia crassipes was carried out through hydrolysis process. Contents of reducing sugar were obtained using Dinitrosalicylic assay method. Reducing sugar contents were found maximum in treated hydrolysate in comparison to untreated hydrolysate in all conditions which was optimized. It was done due to changes in the physical and chemical structure of lignocellulosic biomass through pretreatment. In this study Water Hyacinth was used as feedstock for possible strategies by which biomass of Water Hyacinth was converted to reducing sugar. In hydrolysis, treated and untreated hydrolysate was saccharified by Trichoderma virdae. Saccharification was carried out at optimum comditions such as inoculation period, pH, temperature and substrate concentration. Maximum yield of reducing sugar contents were 565 µg/ml, 528 µg/ml, 475 µg/ml and 415 µg/ml in respected to optimum conditions of inoculation period 7^th days, pH 5.5, temperature 30⁰C and substrate concentration 2.0gm in untreated hydrolysate and 605 µg/ml, 591 µg/ml, 534 µg/ml and 602 µg/ml in respected to optimum conditions of inoculation period 5^th days, pH 5, temperature 30⁰C and substrate concentration 2.0gm in treated hydrolysate. In this paper compression was made between treated and untreated sample. Result showed the potential of Water hyacinth in both treated and untreated conditions for reducing sugar production.

Keywords- Hydrolysate, lignocellulosic biomass, optimization, Saccharification, Water Hyacinth, Feedstock.