Occurrence of Virulence and Antibiotic Resistance Genes in Aeromonas Species Isolated from Diverse Sources

Abstract

Occurrence of transferable or infectious diseases caused by multidrug resistant and inherent virulence properties is of great medical importance, thus becoming a serious or major community and public health concern. The aim of this study was to determine the virulence and antibiotic resistance gene of Aeromonas species isolated from diverse sources. The presence of virulence genes was detected in seven species of Aeromonas such as A. hydrophilla, A. caviae, A. veronii, A. simiae, A. punctata, A. finlandiensis and Plesiomonas shigelloides isolated from diverse sources. The detected virulence factors via polymerase chain reaction using specific primers include heat stable cytotonic enterotocin (AST), heat labile cytotonic enterotoxin, (ALT), cytotoxic enterotoxin (ACT), DNA adenine methyl transferase (DAM), enolase and haemolysin (HlyA) gene, while antibiotic resistance gene of Tet M was amplified at the amplicon size of 200 base pair. This is an indication that these species of Aeromonas are considered to be potential pathogens.

Keywords: Aeromonas species, Antibiotic resistance, virulent properties, diverse sources