Effect of Temperature on Production Intracellular and Extracellular Invertase by Potential Indigenous Strain Kluyveromyces Marxianus Using Sugarcane Molasses

Abstract

This study is focused on enzymolgy regarding enzyme purification technique and application of thermotolerant specie Kluyveromyces marxianus yeast in bio reactions for the research and optimization of fermentation temperature for intracellular and extracellular enzyme production. Fermentation studies were carried out in shake flask level to optimize intracellular and extracellular enzyme production by changing  process conditions like temperature range from (30 to 55⁰C), the pH (5.5), speed (350).  The substrate type sugar cane molasses 15% added as a carbon and ammonium sulphate (0.75%) as nitrogen source. The optimized fermentation temperature was found 45^oC, at pH 5.5, and rpm was 350. The production of  intracellular invertase was (890µmoles/min/g) while the extracellular (120µmoles/min/g) Kluyveromyces marxianus was greater efficiency as compared to other specie because of its metabolic activity, which express more heat stability and Invertase activty upto 65⁰C.

Keywords: Yeast, Fermentation, K.marxianus

DOI: 10.7176/JNSR/9-9-02

Publication date:May 31^st 2019