Isolation and Identification of Bacteria in the Rotifer Mass Culture Medium

Abstract

The objectives of this study are to isolate, to identify and to determine the dominant bacteria in the rotifer (brachionus rotundiformis) culture medium. Stages of the method done were by isolating bacteria in the initial uptake condition and final condition of rotifer population, total bacterial analysis, biochemical tests and water quality. Availability of substrate (raw fish) collected at the base trigger an increase in the number of bacteria to 2.7 X 10⁴ CFU / mL. It is identified that 97 isolates are Halococcus sp bacterial which are kemoheterotrof. The species that are able to survive during the cycle density on rotifer mass culture medium is H. saccharolyticus with a percentage of 54.6%.

Keywords: bacterial identification, rotten fish, halococcus sp., rotifers, water quality