The Effect of Grape Leaf (Vitis vinifera L. )Aqueous Extract on LH FSH, PRO, and PRL Levels in Rabbit Females Having Hyperthyroidism

Abstract

Grape leaf extract (GLE) are very potent antioxidant and exhibit numerous interesting pharmacologic activities, including an antioxidant property, and has been suggested to be of use in treatment of several disease. Our objective are to investigate the protective and therapeutic effect of GLE against levothyroxine sodium – indused hyperthyroidism in rabbit. Femal oryciolagus cuniculus rabbits were divided into four groups: the 1 st group, rabbits were orally administered  (using a feeding solution )with daily dose(5 mL distilled water)for two months, the 2^nd group, the rabbits were treated with levothyroxine sodium (50 µg/kg b.w, body weight )for one month. The 3^rd group, the rabbits were treated with GLE(100mg/mL) for one month and group 4^th the rabbits were treated with GLE(100mg/mL) for two  months  . The level of LH,FSH, PRO and PRL were evaluated in blood. The result indicated that the administration of levothyroxine sodium (50µg/kg b. w) induced a significant decrease in LH, FSH, PRO and a significant increase in PRL. Also the obtained data showed significant increase in LH, FSH, and PRO while PRL  showed significant decrease of treated (G3) which received GLE compared with animal group that received levothyroxine sodium (G2). This is may be the presence of proanthocyanidins and procyanidins which have antioxidant and free radical scavenging activities. The result suggests that grapleaf extract may prevent levothyroxine sodium induced hyperthyroidism.

Keyword: Grape leaf extract(Vitis vinifera L.), Levothyroxine sodium, LH, FSH, PRO, PRL