Screening and Characterization of Biosurfactants Producing Microorganism from Natural Environment(Whey Spilled Soil)

Abstract

Biosurfactant are surface active agent produce by diverse group of microorganism. Disposal of whey is common in India especially in West Bengal, so in our study we collect sample form different whey spilled soil and isolate biosurfactant producing bacteria. Total ten different types of bacteria (APCCS1a-S5b) we have isolate for our study. Among them four are screened by growing them in CTAB agar plates and their hemolytic activity in blood agar plates. We analyses four strains for their ability to produce extracellular biosurfactant by oil spreading technique and finally confirmed by drop collapsing test. The extracellular biosurfactant of the isolated strains were extracted by conventional way and they are characterized by thin layer chromatography (TLC) and were shown that they are glycolipid (APCCS_1a, APCCS_3b and APCCS_5a) and lipopeptide (APCCS_1b) in nature.The surface tension activities of extracted biosurfactant were evaluated by emulsification index (E₂₄). Finally we characterized the isolated strains according to Berge`s Manual and they are belonging to Pseudomonas (APCCS_1a and APCCS_5a), Lactobacillus (APCCS_2b) and Bacillus (APCCS_1b) group.

Keywords: Biosurfactants, Thin layer chromatography (TLC), cetyltrimethylammonium bromide (CTAB), hemolysis, whey, emulsification index.