The Effect of Aerobic Condition and The Incubation Period On the Isolation of Group a Beta Haemolytic Streptococci

Abstract

The effect aerobic, anaerobic condition and the incubation period on the isolation of group A beta haemolytic streptococci investigated. Totally 237 throat culture investigated, 165 (69.6%) consist kinder garden and orphanage and 72 (30.4%) taken from pediatric polyclinic. Culture made on 5% defibrinated sheep blood agar. Cultivation made in two media one of them incubated at 35˚C for 24 and 48 hours. The next put at anaerobic Gas Pack system and incubated for 48 hours. Grouping of the isolates made on subcultures of the isolates by susceptibility of bacitracin and co-trimoxazole.

After 24 hours an aerobic incubation observed group A 24 (10.1%) and after 48 hours this number increased to 37 (15.6%). The isolation rate at anaerobic incubation was 82 (34.6%).

The effect of the anaerobic condition on the isolation of group A beta haemolytic streptococci statistically was significant (p˂0.001). Also the incubation period of aerobic conditions found more effective in 48 hours (p˂0.001).