Investigation of Antibacterial Activity of Various Essential Oils against Seven Different Clinical Isolates

Abstract

In this study, antimicrobial activity of Allium sativum, Aleo vera, Cathamus tinctorius, Eucalyptus spp., Foeniculum vulgara, Jasminum officanalis, Laurus nobilis, Mentha spp., Myrtle spp., Nigella sativa, Rosmarinus officinalis, Salvia spp., Syzgium aromaticum, Thymus spp. Urtica dioica, Zingiber officinale commercial essential oils and various antibiotics was investigated against seven different clinical isolates. For this purpose, disc diffusion and microdilution methods were used to antimicrobial activity assay in the research. Minimum Inhibitor Concentration values of essential oils exhibiting significant antimicrobial activity against clinical isolates were determined. The commercial essential oils used against clinical isolates in the study and the highest inhibition zone values were obtained from black seed cumin essential oil as 39,92 mm for Bacillus cereus isolates, from thyme-2 essential oil as 29,40 mm for Bacillus cereus JCM 2152 isolate, from thyme-1 essential oil as 21,59 mm for Bacillus cereus ATCC 14579 isolate, from thyme-2 as 17,00 mm for Pseudomonas mendocina ATCC 25411 isolate, from mint, clove, eucalyptus-2, sage and laurel essential oils as16,20 mm, 16,00 mm, 14,33 mm, 13,96 mm and 13,25 mm for Bacillus cereus ATCC 14579 and Bacillus cereus JCM 2152, respectively. Most of the antibiotics tested against clinical isolates were either not effective at all or their activities were found to be lower than essential oils. The lowest MIC value obtained in the study was determined as 0.12% (v / v) from thymus-2 essential oil for Bacillus cereus ATCC 14579

Keywords: Antimicrobial activity, 16S rRNA analysis, Clinic bacteria, Essential oil.