A Study on In Vitro Propagation Possibilities of Some Clone Rootstocks of Prunus Species

Abstract

In vitro techniques which is one of the modern breeding methods offer alternative methods to meet the growing food demands in parallel with the increasing world population by allowing rapid and intensive mass propagation of varieties and rootstocks in fruit cultivation under laboratory conditions. Clone rootstocks which are economically important and early yielding with maximum yield per unit area are preferred in fruit production. In this study, the propagation possibilities of Marianna GF 8/1, Myrobolan B, Myrobolan 29-C and St Julien A clone rootstocks used in the production of stone fruit species were investigated. In this study, shoot tips were used as explants. MS (Murashige-Skoog) media was used as the propagation media and 30 g L-1 sucrose and 7 g L-1 agar were added. The shoot tips were planted into the media and then placed in the temperature and light controlled culture chamber. Samples were placed to the subculture at 1-month intervals. Explant length, number of leaves and number of tillings were observed every 20 days. Nine different MS media were tested during the shoot growth stage. The best propagation was obtained in the 1 mg L-1 BAP + 0.5 mg L-1 IBA + 0.25 mg L-1 GA3 media. Eight different MS media were tested during the rooting stage. The best rooting was achieved in a media containing 1/2 MS + 2 mg L-1 IBA.

Keywords: Marianna GF 8/1, Myrobolan B. Julien A, Myrobolan 29-C, micropropagation, plant growth regulators.

DOI: 10.7176/JSTR/5-9-10