Treated with Nitric Oxide Pepper Plant under Drought Stress Proline, Protein Determination of Relative Water Content and Chlorophyll amount

Abstract

The aim of this study was to determine the possible roles of NO (Nitric Oxide) which has the characteristic of messenger molecule in some metabolic changes under the effect of drought stress in plants and to determine the relationship between proline, protein, proportional water content and chlorophyll amounts. The study was carried out under controlled conditions in 16/8 hour light / dark photoperiod, 25 0C and 70% humid climate room. The plants were cultured in containers containing Hoagland nutrient solution. For application of drought stress, 10% Polyethylene Glycol (PEG 6000) was added to the nutrient solution which corresponds to the osmotic potential of -0.40 MPa. Before applying drought stress, different doses of pepper seedlings sodium nitroprusside (SNP) and potassium salt (carboxy-PTIO) were applied externally after SNP 0.01, SNP 1, SNP 100 and SNP 0.01 + cPTIO, SNP 1 + cPTIO, SNP. 100 + cPTIO. Sampling was performed on the 10th day of drought. After the total plant weights were measured, Proline, Protein, Proportional Water Content (OSI), and Chlorophyll content of the leaves of pepper plants were examined in order to clarify the damage mechanism of drought stress and the effect of stress on applications. In terms of total plant weight, it was observed that the growth and growth of pretreated plants were better with 0.01 and 1 doses of SNP and an increase in Proline, Protein, OSI and Chlorophyll amounts.

Keywords: Pepper, Capsicum annum, carboxy-PTIO, Drought stress, Nitric oxide, Proline, Protein, Chlorophyll, SNP

DOI: 10.7176/JSTR/5-10-06