Anticancer Activity of New Di-Nuclear Copper (I) Complex

Abstract

In-vitro biological activities of the free new H₄L ( indole-7-thiocarbohydrazone) ligand and its Ni(II), Pd(II) , Pt(II), Cu(II), Ag(I), Zn(II) and Cd(II)  complexes are screened against two cancerous cell lines, that revealed significant activity only for [Cu₂Cl₂(H₄L)₂(PPh₃)₂]  after 72 h treatment by the highest tested concentrations. The Copper(I) complex was characterized by X-ray Crystallography and the NMR spectra, whereas it has been confirmed to have momentous cytotoxicity against ovarian, breast cancerous cell lines (Caov-3, MCF-7). The apoptosis-inducing properties of the Cu(I) complex have been investigated through fluorescence microscopy visualization, DNA fragmentation analysis and propidium iodide flow cytometry.

Keywords: Cu(I) complex, biological investigation, anticancer activity & DNA fragmentation analysis.