Ciprofloxacin Intercalated with ZnO to Produce a Nanohybrid Used as a Delivery Machine

Abstract

Ciprofloxacin (Cip) with zinc layered hydroxide (ZLH) as the precursors nanohybrid (CipN) was synthesized under an aqueous environment. The synthesis, bioactivity and the anti-bacterial activity of CipN nanoparticles has been evaluated against a wide variety of bacterial strains such as staphylococcus, streptococci, E. coli and hellcobacter. The anti-bacterial activity of CipN has a highly safe compound and may be considered for combination therapy against many bacterial strains, due to its potential synergistic effect with important antibiotics. Powder X-ray diffraction showed that the basal spacing of the nanohybrid was 2.5nm, resulting in the spatial orientation of Cip molecules between the interlayers of ZnO oriented with the direction of the z-axis. SEM images indicate the confirmations of the success of the intercalations. The AFE (Atomic Forcing Electrons) shows the average size of particles found was 86nm. The FTIR study showed that the intercalated CipN spectral feature is generally similar to that of the Cip free molecule, but with bands slightly shifted. This indicates that some chemical bonding of Cip presence between the nanohybrid interlayers was slightly changed due to the formation of host–guest interaction. The nanohybrid is of mesopores type with 54.5% drug loading and enhanced thermal stability. The drug CipN nanohybrid was found to be sustained and therefore has good potential to be used as a nanohybrid drug which is more effective than the free one. The vitro bioassay study showed that the CipN has a mild effect on the hepatocyte cells, more than its counterpart, free Cip.

Keywords: ZnO; nano materials; antimicrobial activity; intercalations.