Using RP-HPLC Method for Determination of Some Anti-Inflammatory (NSAIDs) in Pharmaceutical Drugs

Abstract

Determination of some Anti-Inflammatory (Ketoprofen (KP), Flurbiprofen (FP), and Diclofenac Sodium (DS)) in pharmaceutical drugs were carried out employing Reverse Phase of High Performance Liquid Chromatographic using isocratic separation. Separation was performed on an Enable C18 column (250 mm x 4.6 mm, 5.0 μm) using ((1%) Triethylamine aqueous buffer adjust pH=2 by H3PO4 (85%): Methanol: Acetonitrile); (35: 20: 45 v\v%) as the mobile phase at a flow rate of 2.0 ml/min. The wavelength detection was set at 220 nm. The linearity was observed over a concentration range of (0.05–200) μg/ml for RP-HPLC method (correlation coefficient=0.999). The developed method was validated according to ICH guidelines. The relative standard deviation values for the method precision studies were < 2% and the accuracy was > 99%. The developed method was used successfully for the determination of Ketoprofen (KP), Flurbiprofen (FP) and Diclofenac Sodium (DS) in Tablets and Dry Powder for inject pharmaceutical formulations.

Keywords: Ketoprofen (KP), Flurbirprofen (FP), Diclofenac Sodium (DS), Anti-Inflammatory, RP-HPLC.