The Effect of NADH in the Oxidization of Tetra Methyl Benzidine in the H2O2 /Peroxidase System

Abstract

More than 1000 chemical material, which used in deferent categories, such as (cosmetic, detergent, pesticides, etc) may cause cancer to humane as will as animal, were recognized .

There carcinogens can be divided into two group:

The first group: material causes cancer without enzyme affection.

The second group: chemically inert materials, which exhibit its Carcinogens action after the effect of enzyme during metabolism inside the living body. And such materials are aromatic amines. Metabolized aromatic amines under the effects the enzymes by different path way and  the Peroxidase path is one of these path way, in Which aromatic amines changed from non active into active form, Which can interact with the larges bio molecules as nucleic acids DNA and inhibited it. The aromatic amines metabolized under the effects the enzymes by different path way. and the Peroxidase path is one of these methods, in which aromatic amines changed from non –active into active form ,which can interact with larges biological molecules as nucleic acids DNA and inhibited it, Intra cellular components performed multi- functional such as Glutathione GSH, NADH. It was chosen because of the high damper NADH concentration in the cell (10-3M) and its spread in all types of tissues NADH(H+) inhibit large number of products of oxidation of the  Aromatic amines cancerogenus compounds, such as benzidine Substrate and its derivatives such as3,3',5,5' tetra methyl benzidine  in  Competitive path way. NADH: nicotine amide adenine dinucleotide (reduced form).

Keywords: tetra methyl benzidine, Peroxidase, inhibition, nicotine amide adenine dinucleotide.