Expression and Inhibition of Il-23 by Colon Cancer Cells: A Promising Approach in Prevention of Ibd.

Abstract

Inflammatory bowel disease is chronic uncontrolled inflammation characterized by intense mucosal recruitment of activated leukocytes. Here we have developed an in vitro model for IBD using colon cancer cell line HT-29. Colon epithelial cell line HT-29 expressed elevated IL-23 levels when induced with LPS isolated from various bacterial strains. In this in-vitro model of IBD, IL-23 was playing a key role in inflammation cascade. Action of anti-inflammatory drug Sulfasalizine was studied and was found to block the up regulation of IL-23.

Keywords: IBD (Inflammatory Bowel Disease), HT-29, IL-23 (Interleukin-23), Sulfasalizine, LPS (Lipopolysaccharide).